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BD NIH 3T3 + Vanadate + PDGF Lysate
Useful control for the western blotting application
Supplier: BD 612069
Includes: Mouse Embryonic Fibroblast
Description
- Can be rich in endogenously expressed proteins
- Convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel
Specifications
| Embryonic Fibroblast | |
| 500 μg | |
| Mouse | |
| -20°C |
| NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C | |
| 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol | |
| 1 mg/mL |
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