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BD Mouse Macrophage plus IFNγ/LPS Lysate
Convenient to use and useful control for western blotting application
Supplier: BD 611473
Description
- Source is rich in endogenously expressed proteins
- Provided in denatured format allowing for immediate loading onto SDS-PAGE gel
- Storage buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)
Routinely tested western blot
Specifications
Macrophage | |
500 μg | |
Mouse | |
-20°C |
Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10ng/mL IFNgamma and 1μg/mL LPS for 12 Hours, western blotting of mouse macrophages stimulated with IFNgamma and LPS | |
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol | |
1 mg/mL |
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