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BD Annexin V: FITC Fluorescence Microscopy Kit
Fluorescence staining for apoptosis
Supplier: BD 550911
Includes: Annexin V-FITC, Annexin V Binding Buffer, and PBS Buffer
Description
Early in apoptosis, before the loss of membrane integrity,phosphatidylserine (PS) translocates from the inner to outer leaflet of plasma membrane, exposing it to external cellular environment at surface of plasma membrane. Annexin V is 35-36kDa, calcium-dependent, phospholipid-binding protein with a high affinity for PS and is used to indirectly monitor PS translocation. Annexin V assay is facilitated by the observation that PS translocation appears to be a universal apoptotic phenomenon.
- Binds to negatively charged phospholipid surfaces
- Defined calcium and salt concentrations are required
Used in Fluorescence microscopy
Specifications
| Fluorescence Microscopy | |
| 25 Slides | |
| Fluorescence Microscopy Kit |
| Annexin V-FITC, Annexin V binding buffer, and PBS buffer | |
| 4°C |
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