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IBA LifeSciences Avidin; 50 mg

Supplier:  IBA LifeSciences 20204050


Avidin is a tetrameric biotin binding protein (KD ~1 x 10-14) extracted from egg white. It prevents biotin and biotinylated proteins from binding to Strep-Tactin or Strep-TactinXT, whereas binding of the Twin-Strep-tag and Strep-tagII is not influenced. Biotin and/or biotinylated proteins can potentially reduce the binding capacity of Strep-Tactin as well as Strep-TactinXT and are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag and Strep-tagII fusion proteins from biotin-containing extracts or media are intended to be purified via Strep-Tactin or Strep-TactinXT affinity chromatography, biotin has to be masked by addition of avidin prior to application onto the resin.

Catalog No. NC0058476

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