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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Supplier:  Applied Biosystems™ 4472920

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Catalog No. 44-729-20


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Includes: 2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components.

Description

Description

  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
  • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
  • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:
  • SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
  • It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Liquid
High
AmpliTaq DNA Polymerase
7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
10 x 5 mL
Gene Expression
Built-In Hot Start
DNA (Genomic), cDNA
SYBR
Vial
qPCR
Fast or Standard
SYBR GreenER
SYBR™
UP (Ultra Pure)
100 Reactions of 20 μL
2X
5000 Reactions
10 x 5 mL
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