Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Supplier:  Applied Biosystems™ 4472908

Catalog No. 44-729-08

Includes: 2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components.

Description

• Specific - minimize primer-dimer and non-specific amplification
• Reproducible and sensitive - consistent amplification across a wide dynamic range
• Bright - contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control - contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:

• SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
• The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:

• SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:

• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:

• SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
• It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

Specifications

• Content And Storage: The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  
  See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
• Form: Liquid
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 1 x 5 mL
• Sufficient For: 100 Reactions of 20 μL
• Concentration: 2X
• No. of Reactions: 500 Reactions
• Volume: 50 mL

• Detection Method: SYBR
• Format: Vial
• PCR Method: qPCR
• Reaction Speed: Fast or Standard
• Label or Dye: SYBR GreenER
• Product Line: SYBR™
• Purity or Quality Grade: UP (Ultra Pure)
• Shipping Condition: Wet Ice
• For Use With (Application): Gene Expression
• Hot Start: Built-In Hot Start
• Sample Type: DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.