CD49b Hamster anti-Mouse, PE, Clone: HMA2, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 558759

Catalog No. BDB558759

Description

The HM α 2 antibody reacts with integrin α 2 chain (CD49b), the 150-kDa transmembrane glycoprotein that non-covalently associates with the integrin β 1 subunit (CD29) to form the integrin α 2β 1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets; but it is not on thymocytes or Peyer’s-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HM α 2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HM α 2 mAb blocks the staining of splenic NK cells by the anti-CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858, for the PE conjugate). Therefore, mAb HM α 2 may be used like the DX5 mAb for identification of NK cells.

Specifications

• Antigen: CD49b
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Integrin α2 chain
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: HMA2
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse colon carcinoma cell line Colon26
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ