Cytiva Amersham™ Megaprime™ DNA Labeling System
For labeling DNA probes to specific activities >1 × 109dpm/μg in 10 minutes
Supplier: Cytiva RPN1606
Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25ng substrate DNA.
- Features cloned Klenow fragment and random nonamers in the random-prime labeling technique
- No need to remove unincorporated nucleotides before hybridization
- Protocols for labeling directly from low melting point agarose
|-15°C to -30°C|
|Primer solution 150μL, Labeling Buffer dATP, dGTP and dTTP in Tris HCl pH7.5, 2-mercaptoethanol and MgCl2 300μL, Nucleotide solutions dATP, cCTP, dGTP, dTTP in Tris HCl pH 8, 0.5mM EDTA, Reaction Buffer A 10 x concentra|
|Megaprime DNA Labeling System, dCTP (for use with labeled dCTP)|
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