Cytiva Amersham™ AlkPhos Direct™ ECF Detection Module

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Supplier:  Cytiva RPN3685

Catalog No. 45-000-938

Description

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• ECF detection for quantitation experiments
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm²: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.

Specifications

• Sufficient For: 2500cm2 membrane
• Includes: Sufficient for 25 or 50 labeling reactions and detection of 2500cm² membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
• Quantity: Each