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Cytiva AlkPhos Direct™ Hybridization Buffer for Direct Labeling System

Fast and convenient system for all high-sensitivity applications using DNA, RNA, or oligonucleotide probes

Supplier:  Cytiva RPN3688

Catalog No. 45-000-488

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Includes: Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 5000cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star™ or ECF detection reagents.



  • Hybridization stringency can be controlled by elevating temperatures up to 75°C (167°F), or by varying salt concentration
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF™ detection for quantitation experiments
  • Thirty minutes from completion of hybridization to application of detection reagent: saves two to three hours over conventional indirect methods
  • Resulting probes can be used without purification



Fast, convenient for high-sensitivity applications with DNA, RNA, Oligo probes
Labeling reagent, crosslinker, control DNA, Reaction Buffer, hybridization Buffer, blocking agent, CDP-Star™ or ECF detection reagents
Amersham AlkPhos Direct Hybridization Buffer sufficient for 5000 cm2
For 5000 cm2 membrane


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