BD Pharmingen™ 488 EdU Click Proliferation Kit, 50 Tests
For research use only
Supplier: BD Pharmingen™ 565455
The BD Pharmingen™ 488 EdU Click Proliferation Kit provides a tool for analyzing DNA synthesis in cycling cells. In this method, the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle. EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide. The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU. When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).
The BD Pharmingen™ 488 EdU Click Proliferation Kit contains 6-FAM Azide, which is excited by the blue laser and has an excitation maximum of 496 nm and an emission maximum of 516 nm.
Please note that this kit is provided as Part 1 of 2 (Components A, B, and C) to be stored dry and protected from light at -20°C, and Part 2 of 2 (Components D, E, F, and G) to be stored dry at 2 - 8°C or room temperature.
|Research Use Only|
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